Enviropig DNA Testing Summary

Don Lee, Presenter Author

03/08/2016 Added

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A summary of the fourth step of the Enviropig genetic engineering story For more information visit the Enviropig website: https://ge.unl.edu/enviropig/

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[00:00:11.126]Enviropig learners,
[00:00:12.310]we have now completed step four
[00:00:14.609]in our learning environment
[00:00:16.745]and so you can understand
[00:00:19.392]all of the steps on our screen
[00:00:22.526]and where they help accomplish
[00:00:25.568]the progression of the journey of a gene
[00:00:29.190]to create our final enviropig
[00:00:32.627]so let's summarize our learning here.
[00:00:36.249]In step four,
[00:00:38.106]we learned that you're breeding pigs,
[00:00:40.637]but you want to do careful DNA based diagnostics.
[00:00:44.515]Not just to determine whether the pig
[00:00:46.999]has or doesn't have the transgene,
[00:00:50.087]but you're also interested in creating
[00:00:52.200]homozygous true breeding lines of these pigs
[00:00:56.496]in order to maximize the effectiveness
[00:00:58.771]of the trait or
[00:01:00.884]the reliability of inheritance
[00:01:02.788]so the DNA testing procedures
[00:01:05.412]are a very important and integrated part
[00:01:08.651]of the genetic engineering process.
[00:01:10.857]So if we think about the journey of the gene,
[00:01:12.668]again if the bacteria in the mice
[00:01:14.781]contributed their parts of the gene
[00:01:17.451]to create the transgene,
[00:01:19.633]the transformation specialist introduced that transfene
[00:01:22.698]into pig embryonic cells
[00:01:25.252]and if they were successful,
[00:01:26.622]they could create entire transgenic baby pigs.
[00:01:31.475]Those could be selected for
[00:01:33.007]by using DNA testing
[00:01:35.097]and the individuals that carry the transgene
[00:01:37.349]can be parents in the breeding progress
[00:01:42.876]to create larger families
[00:01:45.244]that carry this transgene
[00:01:47.027]and eventually homozygous families
[00:01:49.906]that will reliably express this transgene
[00:01:54.387]and achieve the desired effect
[00:01:56.384]of having pigs that produce
[00:01:58.358]low levels of phosphorus in their manure.
[00:02:01.260]We've completed the journey of the gene.

Enviropig DNA Testing Summary

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