Enviropig Step 3

Dept. of Agronomy and Horticulture Author

01/26/2016 Added

655 Plays

Description

The third step in the Enviropig story that tells us about how breeders select for the correct traits in the breeding process. For more information visit the Enviropig website: https://ge.unl.edu/enviropig/

Searchable Transcript

Search:

[00:00:06.379](upbeat music)
[00:00:14.989]My name is Amy Desaulniers
[00:00:16.659]and my job is to put transgenic embryos
[00:00:19.147]into the reproductive tract of female pigs.
[00:00:21.499]And then we raise those pigs to sell to producers.
[00:00:26.613]First we put the transgenic embryos
[00:00:28.888]into the female reproductive tract.
[00:00:30.947]And then piglets are born
[00:00:32.208]and we raise those piglets up to adulthood.
[00:00:34.677]We confirm that they are transgenic with a DNA test
[00:00:37.477]and we evaluate the function of the a transgene
[00:00:39.899]and then we make crosses
[00:00:41.917]to produce a family of transgenic pigs
[00:00:44.317]that we can sell at market.
[00:00:46.755]We get the embryos with the transgene from other scientists
[00:00:49.637]who inserted the transgene.
[00:00:51.016]We put the transgenic embryos
[00:00:53.013]into the female pigs during surgery.
[00:00:56.216]And we put about 30 transgenic embryos
[00:00:59.343]into the reproductive tract of females
[00:01:01.786]because they ovulate about 20 eggs.
[00:01:04.074]And they have a litter size of about 15,
[00:01:06.805]so we want to put enough embryos in there
[00:01:09.204]to get a normal litter size.
[00:01:13.515]Once we put the transgenic embryos into the female
[00:01:16.467]it takes about 114 days for piglets to be born.
[00:01:21.473]Once the mom gives birth to a transgenic piglet
[00:01:24.128]we perform a DNA test to confirm that they're transgenic
[00:01:27.229]and then we raise them to adulthood,
[00:01:29.109]which is about 180 days in females and 300 days in males.
[00:01:33.019]With our first transgenic adults
[00:01:35.479]we first make sure they're healthy
[00:01:37.155]and then we evaluate the function of the transgene.
[00:01:40.249]To evaluate the function of the transgene
[00:01:42.090]we compare the heterozygote transgenic pigs
[00:01:45.668]with their litter mate controls.
[00:01:47.783]First we look at their pedigrees
[00:01:49.613]and then we also use our knowledge of Punnett squares
[00:01:52.023]to determine what pigs to cross
[00:01:53.813]if we want to get heterozygote or homozygote piglets.
[00:01:57.053]And if the inheritance pattern was predictable
[00:02:00.563]using a Punnett square,
[00:02:01.483]then we know we've made the right pigs.
[00:02:05.151]The pigs for market will be homozygous
[00:02:06.960]because they have more dramatic phenotype.
[00:02:09.522]Since they're homozygous
[00:02:10.963]they produce more of the transgene
[00:02:12.213]so that's why we have a more dramatic phenotype.
[00:02:15.160]A sow can have approximately 2.5 litters a year
[00:02:18.153]and that equates to about fifteen piglets per litter
[00:02:21.746]so she can have around 30 piglets a year.
[00:02:25.543]We take about three years of breeding
[00:02:27.535]to get an acceptable group of transgenic pigs
[00:02:30.318]that we could market.
[00:02:34.199](upbeat music)

Enviropig Step 3

Description

Searchable Transcript

Comments

Related Channels

Enviropig Step 3

Description

Searchable Transcript

Comments icon comment

Related Channels

Comments