Pollen-Mediated Gene Flow and Transfer of Herbicide-Resistance Alleles
Amit Jhala, Professor/Extension Weed Management Specialist, Department of Agronomy and Horticulture, UNL.
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10/07/2024
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Description
Managing herbicide-resistant weeds is a challenge for growers. Once herbicide-resistant weed evolve, it can be spread by pollen, known as pollen-mediated gene flow. Reproductive biology differs markedly among weed species, as does the potential for gene flow. Pollen-mediated gene flow at a distance could allow the spread of rare herbicide resistance alleles and favor the evolution of multiple herbicide-resistant biotypes through the accumulation of resistance genes in an individual weed or weed population. The speaker will discuss examples of economically important weeds such as waterhemp, palmer amaranth, and giant ragweed and crops such as corn and popcorn to demonstrate the role of pollen-mediated gene flow in transfer of herbicide-resistance alleles. For more information about speaker’s research program: https://agronomy.unl.edu/jhala.
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- [00:00:00.740]The following presentation is part of the Agronomy and Horticulture Seminar Series
- [00:00:05.760]at the University of Nebraska-Lincoln.
- [00:00:07.360]All right, we've got 11 o'clock.
- [00:00:10.960]Thanks everybody for coming today.
- [00:00:14.000]It's a treat for me to introduce Amit.
- [00:00:20.160]We started around the same time here, so a little over a decade ago.
- [00:00:25.400]Dr. Amit Vahallan will talk to us today about gene, all-immediate gene flow, and transfer
- [00:00:30.680]of herbicide-resistant alleles.
- [00:00:32.000]For the few that don't know Amit, he's our associate department head, as well as professor
- [00:00:39.120]and extension management specialist.
- [00:00:40.120]I'm excited to have Amit kick off our fall seminar series, and for those that are here,
- [00:00:47.620]and unfortunately for those that are online, Amit brought ice cream in the back, and there's
- [00:00:51.840]some other snacks and goodies.
- [00:00:52.840]Feel free to treat yourself.
- [00:00:54.840]The goal is to treat yourself.
- [00:00:55.360]This is the bucket to be gone, I think is what I heard, but otherwise I'll turn it over
- [00:00:59.400]to you, Amit.
- [00:01:00.400]Thanks so much for picking it up.
- [00:01:02.400]Please join me in welcoming Amit.
- [00:01:03.400]Okay.
- [00:01:04.400]Well, thank you very much for joining us today, and thanks DIREC and seminar committee for
- [00:01:09.240]an opportunity to speak here.
- [00:01:11.920]When DIREC contacted me, I was thinking like when I gave my last seminar in the department
- [00:01:16.340]and it was almost like before 10 years, so in the last 10, 12 years, our lab did a lot
- [00:01:23.320]of studies on gene preference.
- [00:01:25.320]And so I thought this will be a good opportunity to summarize whatever we did on this topic
- [00:01:35.080]and also the progress made by all our graduate students in the lab.
- [00:01:40.620]So yeah, thanks for being here and we'll start the journey.
- [00:01:44.460]As you know, weed management is a permanent challenge, right?
- [00:01:48.820]Weeds grow every single year, unfortunately, compared with insects.
- [00:01:55.280]Insect and disease where you have an option like usually insect and disease pressure do
- [00:02:00.020]not come every single year compared with weeds are kind of every year because we have a lot
- [00:02:05.640]of weed seed bank in the soil that comes keep coming up emerging from the soil whenever
- [00:02:12.720]they will get an opportunity to emerge, they just need a little bit of moisture and little
- [00:02:18.080]bit of sunlight and that's all.
- [00:02:21.340]So for weed management, herbicides have been used quite
- [00:02:25.240]a bit, especially in commercial corn and soybean fields because agriculture is at very
- [00:02:32.020]large scale here in North America.
- [00:02:36.320]So herbicide discovery started during the World War II, around 1940s.
- [00:02:42.420]And after World War II, the first herbicide came to the market was 2,4-D for control of
- [00:02:47.760]broadleaf weeds and after that, a lot of new chemistry came to the market with different
- [00:02:55.200]herbicide target sites, amino acid biosynthesis and chlorophyll biosynthesis and there are
- [00:03:00.300]so many of them.
- [00:03:02.120]And at the same time, the use of herbicide increased and so do the number of weeds evolve
- [00:03:08.620]resistance to herbicides.
- [00:03:10.980]So this is the total number of weeds which are, as of now, 532 weed biotypes are resistant
- [00:03:19.540]to at least one herbicide.
- [00:03:21.820]Some of them are resistant to even multiple herbicides.
- [00:03:25.160]So, this is just an overall number of weeds resistant to herbicides and about 150 are
- [00:03:32.640]dicot weeds and about 100 are monocot weeds among that total.
- [00:03:42.980]Before I start, I just would like to start with this quote by Albert Einstein.
- [00:03:47.640]He says, "Imagination is more important than knowledge because knowledge is limited to
- [00:03:52.540]what we now know and understand.
- [00:03:55.120]Science can embrace the entire world."
- [00:03:58.280]And that is also true in agriculture, right?
- [00:04:01.040]Because see, Russell Carson, she was a marine biologist working somewhere in Florida.
- [00:04:07.040]She wrote a book called Silent Spring.
- [00:04:11.040]This was a very controversial book at that time because that book basically describes
- [00:04:16.840]the negative consequences of agricultural pesticides being used at a commercial scale.
- [00:04:23.420]But in the last chapter of her book.
- [00:04:25.080]She wrote a section about how best we can find out a biological solution of agriculture
- [00:04:32.180]pesticides.
- [00:04:33.180]And I believe this is the biological solution, right?
- [00:04:36.880]Transgenic crops or Bt crops or herbicide resistant crops, whatever you call them, is
- [00:04:42.320]a one step towards the finding out a biological solution of agriculture pesticides.
- [00:04:49.180]We can say, especially when herbicide resistant crops came to the market.
- [00:04:55.040]First, glyphosate resistant soybean came to the market in 1996.
- [00:04:59.720]And just next year in 1997, glyphosate resistant corn came to the market.
- [00:05:04.900]And that is our major cropping system, not only in Nebraska, but also in entire Midwestern
- [00:05:11.220]U.S., right?
- [00:05:12.220]Corn after soybean and soybean after corn and sometimes corn after corn for several
- [00:05:16.220]years in Nebraska irrigated fields.
- [00:05:19.840]So unfortunately, we were relying too much on glyphosate right at that time.
- [00:05:25.000]From the aerial view, you can see this field has some weeds, means this field was not having
- [00:05:33.540]glyphosate resistant corn or soybean, but rest of them looks clean.
- [00:05:36.880]So this system was great.
- [00:05:39.320]Glyphosate is like a once in a century herbicide.
- [00:05:42.660]This type of molecule do not come very often because of number of characteristics.
- [00:05:48.660]Glyphosate can kill more than 300 weed species, including perennial weeds, because it is a
- [00:05:53.760]translocated type of herbicide.
- [00:05:54.960]It can even enter in the root system of the weed and it can kill the weed compared with
- [00:06:01.360]several other contact herbicides that cannot provide that level of weed control.
- [00:06:07.840]So flexible application timing.
- [00:06:09.580]As long as your crop is resistant to glyphosate, you can apply them multiple times in a growing
- [00:06:14.200]season and it is easy to use less expensive herbicide, right?
- [00:06:19.060]Right now, there is no patent on glyphosate, so a lot of generic products are available
- [00:06:24.000]in the marketplace.
- [00:06:24.920]I would say right now, at least there are 400 glyphosate based products are available
- [00:06:30.260]in the marketplace and it is very, very less expensive.
- [00:06:34.040]It might cost around $10 per acre to spray herbicide like glyphosate compared with if
- [00:06:40.420]you want to spray some pre-emergence residual herbicides, it can cost somewhere between
- [00:06:46.180]$30 to $60 per acre, depending on which herbicide you select.
- [00:06:50.900]So it was very affordable for growers and that made it.
- [00:06:54.880]A very success story behind the use of glyphosate for last several years.
- [00:07:01.260]But the problem is we have been relying on glyphosate for so long, right?
- [00:07:06.500]And for so many years, we were switching the crop from corn to soybean, but the herbicide
- [00:07:13.000]was still the same glyphosate and a lot of advertisement efforts from companies also
- [00:07:20.100]made it very popular choice among growers.
- [00:07:23.660]And at the same time.
- [00:07:24.840]When glyphosate was evolving at the same time, the agriculture was also evolving, right?
- [00:07:30.740]A lot of growers have started no-till agriculture, conservation agriculture, which is great for
- [00:07:36.720]a number of reasons like reduce the soil erosions and a number of other soil health benefits.
- [00:07:42.960]But the major limitation of no-till cropping system is you have to rely only on herbicides
- [00:07:49.300]for your weed management program, right?
- [00:07:51.660]So that was kind of a major limitation of no-till.
- [00:07:54.800]No-till cropping system and that also increased the use of herbicide like glyphosate because
- [00:08:00.240]it was very simple to use and very affordable.
- [00:08:05.060]So this is what we are now.
- [00:08:09.300]This is the use of glyphosate every single year went really high.
- [00:08:14.940]If you see this yellow is corn and green is soybean.
- [00:08:18.300]So if you combine these two, since 2008, we have been using like
- [00:08:24.760]almost more than 200 million pounds of glyphosate per acre every single year, which is like
- [00:08:33.660]too much glyphosate that goes in the environment, right?
- [00:08:37.980]And that's why we have now weeds started evolving resistant to glyphosate.
- [00:08:44.140]So far, we have about 50 weeds now resistant to glyphosate worldwide.
- [00:08:51.840]I think in the USA, we have around 32 glyphosate
- [00:08:54.720]resistant weeds across the country.
- [00:08:59.840]In Nebraska, we have six weeds resistant to glyphosate so far.
- [00:09:04.960]And we might have more in future.
- [00:09:08.320]And not only glyphosate, but weeds
- [00:09:10.360]can evolve resistant to any herbicide
- [00:09:12.900]if it will be used repeatedly in the same field.
- [00:09:16.360]So nothing is wrong with the one chemical.
- [00:09:19.500]But even right now, we have, for example,
- [00:09:21.780]palmer amaranth resistant to glyphosate and a number
- [00:09:24.420]of--
- [00:09:24.680]ALS-inhibiting herbicides, atrazine.
- [00:09:27.500]We have been using it for the last almost 45 years.
- [00:09:30.920]We have glyphosate resistant, and we
- [00:09:33.380]have atrazine-resistant waterhemp and palmer amaranth.
- [00:09:36.260]And a lot of them are even multiple herbicide resistant.
- [00:09:42.440]So what is gene flow?
- [00:09:43.480]So there was a lot of debate about gene flow
- [00:09:47.140]and some of the consequences it may create, especially
- [00:09:51.400]at the time when transgenic crops were under development.
- [00:09:54.640]And some articles were written in some journals,
- [00:09:58.780]and a lot of books have been written, especially
- [00:10:01.540]about the gene flow from genetically modified plants.
- [00:10:04.760]What will happen if we insert the good genes
- [00:10:07.980]by using biotechnological approaches?
- [00:10:09.960]And then those genes will go to the weed species
- [00:10:12.660]or closely related species, right?
- [00:10:15.600]Each and every crop has some closely related species.
- [00:10:18.940]For example, if it is sorghum, then it
- [00:10:21.420]has Johnson grass and shatter cane are closely related species.
- [00:10:24.600]So what will happen if you will insert a good gene in sorghum
- [00:10:30.840]and if it will go to those closely related species?
- [00:10:34.080]So there was a lot of debate and a lot of research
- [00:10:36.740]has been done on this topic.
- [00:10:38.020]I was a PhD student at University of Alberta
- [00:10:44.680]in Canada.
- [00:10:45.920]And at that time, I was a part of the project
- [00:10:50.800]for risk assessment of genetically engineered flecks
- [00:10:54.560]in Canada.
- [00:10:55.940]And so at that time, I conducted a study
- [00:10:58.460]about pollen-mediated gene flow in flecks.
- [00:11:01.360]Flecks is an oilseed crop which is not very popular in the US.
- [00:11:06.480]But in Canada, it is a very important crop,
- [00:11:09.200]particularly in Western Canada.
- [00:11:11.220]The states like Saskatchewan, Alberta, and Manitoba
- [00:11:15.100]are very big producers of flecks.
- [00:11:19.740]In fact, Canada is producing about 50% of oilseed flecks
- [00:11:24.020]being produced.
- [00:11:24.520]In the whole world.
- [00:11:26.480]So flecks is an important crop.
- [00:11:28.280]And I was very fortunate to be a part of this project.
- [00:11:31.840]So this was the beginning for me to think about this pollen
- [00:11:35.980]mediated gene flow.
- [00:11:37.480]And that is how I had some level of expertise
- [00:11:41.460]to do this type of studies.
- [00:11:44.140]And then when I came here in Nebraska,
- [00:11:46.660]waterhemp was kind of evolving resistant to ALS
- [00:11:51.460]inhibiting herbicides and glyphosate.
- [00:11:54.480]So this is the soybean field.
- [00:11:56.640]But it looks more like a waterhemp field, right?
- [00:11:59.480]Because there are more waterhemp plants than soybean.
- [00:12:02.280]And this is just a random photograph
- [00:12:05.280]from a field in Nebraska.
- [00:12:07.240]So this is not like something somewhere else.
- [00:12:11.160]Then we did a lot of studies on waterhemp.
- [00:12:13.740]I love this weed because when I started,
- [00:12:17.080]we did a lot of studies, bring a lot of funding,
- [00:12:19.440]and brought more than 25 papers only on this weed to study about.
- [00:12:24.440]Management and emergence periodicity and biology and reproductive biology of this weed.
- [00:12:30.240]So this weed has given me like promotion and tenure just within five years.
- [00:12:35.740]So can't miss that, right?
- [00:12:39.900]So once the field has herbicide resistant weed,
- [00:12:43.320]there are different two ways it can spread resistance from one field to another field
- [00:12:49.260]and maybe one county to another county, right?
- [00:12:52.820]So seed movement is very common.
- [00:12:54.400]Right?
- [00:12:55.400]For example, in this field, if grower will now go to combine this field at the end of
- [00:13:01.920]the season to harvest some grain, he's also going to harvest all the weed seeds in the
- [00:13:08.400]combine.
- [00:13:09.400]And when he will drive across the field, you are basically spreading all the resistant
- [00:13:14.460]weed in your field.
- [00:13:16.420]And when you will take the same combine without cleaning to your another field, you are kind
- [00:13:21.940]of introducing your resistant weed.
- [00:13:24.360]In another field, and when your neighbor will borrow your field, you are giving your
- [00:13:29.180]problem to your neighbor, right?
- [00:13:31.120]So this is how seed movement is very common.
- [00:13:34.320]And it is happening everywhere.
- [00:13:39.140]Something which was very less known was pollen made it a gene flow because we don't see with
- [00:13:44.000]our eyes, right?
- [00:13:45.000]But pollen movement happens everywhere in the nature.
- [00:13:49.140]And pollen is moving since the existence of first flowering plant on the planet.
- [00:13:54.320]And it is also true for the weed species.
- [00:13:56.700]Weed can also do flowering and in case of waterhemp and palmer amaranth, in fact, it
- [00:14:02.020]is more problematic because those are dioceous species means male and female plants are separate.
- [00:14:08.560]So the only way for them to survive is just through the gene flow.
- [00:14:15.460]And another problem is both of these pigweed species are very prolific seed producer.
- [00:14:24.280]For example, this single female plant of Palmer eminence can produce almost half a million seeds per plant.
- [00:14:31.120]So if you think about if you have just like 10 plants, female plants in your field at the time of the harvest.
- [00:14:38.280]It can produce so many seeds and those seeds will go back to the soil and then you have this problem for the next five years, because unfortunately those seeds can remain viable in the soil for like five to six years, and they will emerge whenever there is an opportunity.
- [00:14:54.240]That's why this waterhemp and palmer eminence became very widespread because of all these excellent characteristics that they do pose.
- [00:15:04.240]And they are very small seeded.
- [00:15:06.240]If you can see the comparison of corn seed is almost about 0.6 to 1 centimeter long and these are the waterhemp seeds you can see they are hardly one millimeter long.
- [00:15:20.240]So very small seeded and it is hard to find even when they are in the soil.
- [00:15:24.200]And it is hard to believe when the small seed will grow up, it can become a plant like this big, which can produce like half a million seeds.
- [00:15:35.200]So waterhemp, for example, similar to Palmer emmerons, they both are like obligate outcrossing species.
- [00:15:42.200]This is a male plant and this is a female plant.
- [00:15:45.200]So, for example, if male plant is resistant to some herbicide and if it will outcross this female plant,
- [00:15:54.160]it is giving the resistant trait to the female plant.
- [00:15:57.160]So next year when the seeds will produce from the female plant, they will also be resistant to whatever the trait that male plant was resistant to.
- [00:16:06.160]Because unfortunately, the herbicide resistant trait is a dominant allele.
- [00:16:11.160]So it will dominate whatever the character you have with the susceptible plant species.
- [00:16:17.160]For example, this is the field when soybean is almost ready to harvest, very mature.
- [00:16:24.120]Soybean pods, you can see on the plant.
- [00:16:27.120]And at the same time, we have seen some female waterhemp plants nearby.
- [00:16:35.120]Two female plants, and there was no any male plant nearby.
- [00:16:40.120]So we were wondering like how they can produce seeds without any male plant, right?
- [00:16:45.120]How they will receive the pollen.
- [00:16:47.120]And that was, I believe, just this is the result of the pollen-mediated gene flow.
- [00:16:52.080]And that's time in 2022.
- [00:16:54.080]We thought like we may need to do some study on pollen-mediated gene flow.
- [00:17:00.040]Because this is clearly the case of long-distance pollen movement that must be coming from some male plant in surrounding area.
- [00:17:08.040]Because otherwise this female plant should not produce seeds, right?
- [00:17:11.040]The plant can be there, but if it will not get any pollen, it usually do not produce seeds.
- [00:17:19.040]So we just wanted to do some studies and I was always interested.
- [00:17:24.040]in this topic, but now we have a problem and I had a little bit of expertise on doing this type of study.
- [00:17:31.000]My work was more in flax, but the same concept.
- [00:17:34.000]We were applying to know how far the pollen can move under the field condition.
- [00:17:40.000]So we did this study, gene flow study is a long process.
- [00:17:45.000]You have to start with problem identification.
- [00:17:48.000]We already identified the problem, assuming like gene flow is occurring.
- [00:17:54.000]At the landscape level in Waterhemp.
- [00:17:56.960]We did field studies. We have to do the screening part.
- [00:17:59.960]We have to fit the model and then we need to make the prediction.
- [00:18:03.960]So it's a step by step process that we have to go through to conduct each and individual gene flow studies.
- [00:18:10.960]So this is an aerial view of this field.
- [00:18:14.960]And thank you, Dr. Ferguson. He took this photograph when he was flying his drone in our field.
- [00:18:20.960]This is the field at South Central Ag Lab.
- [00:18:23.960]So here you can see in the center of this field, we transplanted.
- [00:18:31.920]Here is the center of this field. This was about 10 meter diameter.
- [00:18:36.920]We transplanted glyphosate resistant waterhemp plants in the center.
- [00:18:41.920]And then in the surrounding area, in all the cardinal and aldenal directions, we transplanted glyphosate susceptible plants.
- [00:18:49.920]So resistant plants were here and susceptible plants were here.
- [00:18:53.920]They were act as a pollen donor block.
- [00:18:56.920]So this one was giving pollen to these susceptible plants at different distances in all the directions.
- [00:19:05.920]And we were removing all the male plants from here because we wanted to see the pollen movement that comes from these resistant plants, not from the susceptible plants.
- [00:19:16.920]And we were removing all the other weed species.
- [00:19:19.920]And at that time, we really haven't had this waterhemp at all.
- [00:19:23.880]At our South Central Ag Lab, and we were removing some other waterhemp plants from the nearby fields, if we see any.
- [00:19:33.840]And at the end of the season, we were harvesting all the seeds from these female plants of waterhemp at the end of the season.
- [00:19:42.840]So this is how we started transplanting all the plants.
- [00:19:45.840]And it is a lot of work. Actually, gene flow studies are not easy.
- [00:19:49.840]You can see all the students and me were transplanting individual plants.
- [00:19:53.840]And when I was seeing this photo, you may feel like, do we have a dress code to go in the field or what?
- [00:20:01.800]Because see, everybody is in like a yellow jacket and blue jeans.
- [00:20:05.800]And yeah, we don't really have any dress code.
- [00:20:08.800]But on that day particularly, it was like a little bit of raining and we were running late.
- [00:20:13.800]So we wanted to move forward anyhow. So we just picked this yellow jacket from our South Central Ag lab.
- [00:20:19.800]We have different size of this rain jackets.
- [00:20:23.800]We just put it on and we were working on and somehow everyone was in blue jeans on that day.
- [00:20:30.760]So it looks like a dress code for the lab.
- [00:20:34.760]But it was a great coincidence.
- [00:20:36.760]So at the end of the season, we collected all the seeds from the female plants of all the susceptible plants from different distances, all the directions.
- [00:20:48.760]Because we wanted to see whether wind is playing a role to move the rain.
- [00:20:53.760]So we did a lot of greenhouse screening by spraying glyphosate.
- [00:20:59.720]So if a plant will die, it means the plant was susceptible.
- [00:21:03.720]And if plants will survive at a higher rate of glyphosate, it means those plants are resistant.
- [00:21:08.720]They must have received the pollen from the resistant plants that we transplanted in the center of the field.
- [00:21:15.720]And we did a lot of modeling work by using some equations.
- [00:21:23.720]And logic transformation and stuff. I'm not going too much in detail.
- [00:21:27.720]We use this ACHI information criteria to select the best model which comes with the lowest value of AIC.
- [00:21:37.720]And based on this, this was our result. So like the nearer distance, like 0.1 meter, the gene flow was about 50 percent.
- [00:21:46.720]And when we put some susceptible plants in the center block, we had like about 77 percent of out-crossing.
- [00:21:53.680]Our maximum distance was 50 meter from the pollen source, and we still were able to see about 5 percent of out-crossing at 50 meter distance.
- [00:22:06.680]And it was never zero. So that means this gene flow can occur even more than 50 meter, right?
- [00:22:13.640]Because that was our maximum distance at the field level. But this pollen can move and it can out-cross even more than 50 meter.
- [00:22:23.640]And this 5% out-crossing at 50 meter is also a very big number, actually.
- [00:22:28.600]If you think about at landscape level, if 5% out-crossing is happening by receiving the pollen which is resistant to glyphosate.
- [00:22:36.600]So that is how you can little bit of predict this pollen can move and it can really out-cross and spread the resistance.
- [00:22:44.600]So that was our hypothesis that we were able to prove here. Like, yes, pollen can move the resistant allele and it can out-cross.
- [00:22:53.600]In year two study, we repeated everything and we were able to see even little bit of more gene flow at the longest distance.
- [00:23:01.600]It was 9% of out-crossing.
- [00:23:06.600]And we also did some wind roses just to think about the wind can move the pollen.
- [00:23:13.600]And we were able to see more out-crossing in the area where wind was blowing.
- [00:23:17.600]So that means from the center, when wind blows in this south direction,
- [00:23:23.560]we have seen more out-crossing.
- [00:23:25.560]So wind is also playing a role to disseminate this resistant trait.
- [00:23:31.560]And based on our prediction model, we were able to figure it out.
- [00:23:35.560]Like if you want to reduce the gene flow by 50%,
- [00:23:39.560]you have to maintain like 1.5 meter distance from the resistant plant.
- [00:23:44.560]And if you want to reduce like 90%,
- [00:23:46.560]then you have to maintain like 58 meter distance from the pollen source.
- [00:23:53.520]We also did some molecular studies just to make sure
- [00:23:57.520]the herbicide screening we are doing is also matching with our molecular test
- [00:24:02.520]just to make sure we are not any falsely identifying any false positive.
- [00:24:07.520]So we did some molecular studies because when plant is resistant to glyphosate,
- [00:24:14.520]it will have more copy number of EPSPS gene.
- [00:24:17.520]So for example, this is resistant waterhemp,
- [00:24:20.520]which had copy number of about five.
- [00:24:23.480]And on an average, some of them had even like up to 12.
- [00:24:27.440]And when we had an F1 population,
- [00:24:29.440]means the population that we harvested from the field
- [00:24:32.440]had on an average of about four EPSPS gene copy number.
- [00:24:37.440]That means it was 100% resistant to glyphosate.
- [00:24:41.440]And that came from the resistant population we transplanted in the center of the field.
- [00:24:51.440]So we had some of the questions like
- [00:24:53.440]nearer distances, we were in the impression like
- [00:24:56.440]the 100% plants should be resistant, right?
- [00:24:59.440]Because that was the resistant pollen was the only available.
- [00:25:03.440]But the thing is when it's species like waterhemp and Palmer amaranth,
- [00:25:08.440]they are like obligate out-crossing diocese species,
- [00:25:11.440]means they really do not follow the Mendelian pattern of inheritance.
- [00:25:17.440]That was the number one factor why we don't see like 100% of out-crossing.
- [00:25:21.440]The second point could be like
- [00:25:23.400]we might have some glyphosate resistant parent population,
- [00:25:26.400]might have some susceptible population.
- [00:25:29.400]So when you get the allele from susceptible plant,
- [00:25:33.400]then you will end up with having susceptible plant, right?
- [00:25:36.400]So it will not be resistant.
- [00:25:41.400]And we might have some minimum level of some distant natural population of susceptible
- [00:25:47.400]that might have done some out-crossing.
- [00:25:50.400]That could be the reason.
- [00:25:53.360]There is also a chance of apomatric seed production in this diocea species.
- [00:25:58.320]There are some studies have been done which says like the female plant can produce seeds
- [00:26:03.320]even without receiving pollen from the male plant.
- [00:26:08.320]The work has been done in waterhemp particularly.
- [00:26:13.320]So it can produce seeds only in few cases.
- [00:26:17.320]So that could be the reason why we were not able to see like 100% of resistant population
- [00:26:23.320]even in the nearer distance from the pollen source.
- [00:26:28.280]So we have published this paper at that time
- [00:26:32.280]and we were famous for a good reason
- [00:26:35.280]because this was the first study done at landscape level
- [00:26:38.280]and we have given number of interviews in KRVN radio and stuff.
- [00:26:45.280]This was my PhD student Debalin Sarangi.
- [00:26:48.280]He was leading this project and now he is a faculty member at University of Minnesota
- [00:26:53.280]so this was a basic work that we started.
- [00:26:56.240]So now we had a protocol ready to go that we can apply in some other weed species.
- [00:27:01.240]So this is how this started.
- [00:27:04.240]The waterhemp resistant to glyphosate was first identified in Missouri in 2005.
- [00:27:12.240]And then within few years it was almost everywhere.
- [00:27:18.240]So in lot of cases it was like independent evolution.
- [00:27:23.240]It was just based on the history of using glyphosate repeatedly, but not in all the cases.
- [00:27:30.200]And so we were able to believe like at 50 meter distance, if we have seen like 5% and 9% of outcrossing,
- [00:27:38.200]means the pollen is moving through the activity of outcrossing, through the activity of wind, through the activity of pollinators.
- [00:27:46.200]And so pollen is also playing an important role to disseminating this resistant population.
- [00:27:53.200]Because there were some instances where we collected seeds from the field and Grover was really progressive.
- [00:28:00.160]He said, I really didn't use glyphosate in like last five years.
- [00:28:04.160]And although he was seeing glyphosate resistant waterhemp in this field.
- [00:28:09.160]So it was clearly could be the reason of the pollen mediated gene flow,
- [00:28:14.160]because his neighbor might have resistant population and the pollen flow from his neighbor's field to his field.
- [00:28:21.160]And then that is how he ended up.
- [00:28:23.160]With having resistant population.
- [00:28:25.120]So that was our point here, like seed is definitely playing an important role to move the resistance.
- [00:28:32.120]But pollen is also playing an important role, especially if you have a species like waterhemp, which is obligate outcrossing species.
- [00:28:43.120]Now, another problem of amaranthus species is there are about 75 total species in amaranthus, including waterhemp.
- [00:28:53.120]So, for example, this is waterhemp, this is palmer amaranth, and this is a hybrid between them.
- [00:29:02.080]So, if you see the phenotypes of this plant, the leaves are looking more like waterhemp, and the length of petiole was just like palmer amaranth,
- [00:29:13.080]where the length of petiole means this part, which is connecting leaf with the main stem is known as petiole, which is usually very longer.
- [00:29:23.080]So sometimes we are seeing like a hybrid plants that doesn't look like waterhemp, they don't look like palmer amaranth.
- [00:29:31.040]So those are could be the result of the out crossing between them, which is not very common, but it can happen.
- [00:29:38.040]And these are the field. This is something collected from the field photos.
- [00:29:42.040]You can see there is a waterhemp plant nearby and we were able to see this hybrid plant.
- [00:29:47.040]So sometimes even in the nature, you will see some of those hybrid plants.
- [00:29:53.040]So the next project, next student, right?
- [00:29:58.040]So now we wanted to see whether waterhemp can outcross with Palmer emirate.
- [00:30:05.040]Now we had a recipe of the protocol. We put
- [00:30:12.040]HPPD inhibitor resistant waterhemp as a pollen donor in the center of the field and in surrounding area.
- [00:30:19.040]We transplanted HPPD inhibitor
- [00:30:23.000]to a susceptible Palmer emirate. So HPPD is a group of herbicides.
- [00:30:27.000]So before few years we had a herbicide like Calisto and Laudis and impact and
- [00:30:34.720]And armazen, those are very good example of corn herbicides that belong to HPPT group of herbicides.
- [00:30:40.040]So we had some waterhemp resistant to that group of herbicide.
- [00:30:45.040]So we did this study, did a lot of screening part and everything.
- [00:30:49.360]And we were able to see at least about 1% of outcrossing at a nearer distance.
- [00:30:56.340]And it was about 0.001% at a longer distance from the pollen source, where more detail is already in this paper.
- [00:31:05.540]So it was also a little bit of surprising to me, like interspecific hybridization is also happening in the nature at a little bit of higher rate than what we thought earlier it used to be.
- [00:31:20.760]And the practical implications.
- [00:31:26.260]The application of those studies is we started providing information in our field days about how to identify male and female plants of waterhemp and palmer amaranth.
- [00:31:36.160]And there are a number of articles we had published in this Nebraska Farmer is an agriculture magazine.
- [00:31:42.760]Because our idea was we can educate growers if they can identify male and female plants.
- [00:31:48.500]And if it is possible just to chop the female plants before they produce seeds, they can reduce the overall weed seed bank.
- [00:31:56.160]In the soil.
- [00:31:57.220]Because weed like waterhemp and palmer amaranth cannot be just controlled by applying herbicide.
- [00:32:02.980]But our overall message to the grower is now we have to think how best we can reduce the overall weed seed bank of these weeds.
- [00:32:11.680]What best you can do to reduce whatever the seeds going back to the soil.
- [00:32:15.980]Because then you have to deal with them relatively less in next few years.
- [00:32:20.660]So this was a one step in that direction.
- [00:32:24.040]And I'm happy to...
- [00:32:26.140]I'm happy to say that a lot of growers now in Nebraska, they know how to identify between male and female plants.
- [00:32:31.100]And they are doing their best.
- [00:32:32.800]Some of them have started using inter-row cultivator just to chop those plants which are in between the rows.
- [00:32:40.040]When they cannot control very easily with herbicide because both of them have resistance to multiple herbicides.
- [00:32:48.500]The next weed was giant rugweed, which is also a broadleaf weed.
- [00:32:55.520]It's a broadleaf weed.
- [00:32:55.800]It can grow really tall.
- [00:32:57.340]For example, in this photograph, you can see this giant rugweed can grow as tall as like 10 to 12 feet.
- [00:33:03.480]And another problem of this ambrosia species weed is they are prolific pollen producer.
- [00:33:10.600]So when they produce more pollen, means there might be more chances of pollen-mediated gene flow, right?
- [00:33:16.320]So one more student, one more study.
- [00:33:18.640]Same concept.
- [00:33:20.340]We are again back to the square design.
- [00:33:22.900]We know the protocol and the procedure.
- [00:33:25.460]So we transplanted glyphosate-resistant giant rugweed in the center of the field and glyphosate-susceptible giant rugweed in all the directions, up to like 0 to 50 meter distance in each and every direction, all four cardinal and four ordinal directions.
- [00:33:43.960]And in giant rugweed, we were able to see 43 to 60 percent of outcrossing at 0.5 meter distance from the pollen source.
- [00:33:55.120]In like about 3 to 4 percent of outcrossing at 50 meter distance from the pollen source, because they are prolific pollen producer.
- [00:34:04.620]During the pollination period, giant rugweed can produce like a one million pollen grain every single day.
- [00:34:12.540]And that's why you might have seen a lot of people have this pollen allergy.
- [00:34:16.400]And that's actually coming from this giant rugweed.
- [00:34:19.140]And another species is common rugweed, belongs to the same ambrosia.
- [00:34:24.780]So we have published this paper.
- [00:34:30.680]Zahur Gani was the PhD student leading this project.
- [00:34:34.720]And now he's a global R&D scientist with herbicide discovery in FMC.
- [00:34:40.300]So along with our studies, I was glad to see the progress made by our graduate students after graduation.
- [00:34:48.080]This is Johnson grass.
- [00:34:54.440]A perennial weed, which is also a closely related species of sorghum, right?
- [00:35:00.240]Sorghum Johnson grass is like helipens and sorghum is like bicolor, but it can outcross.
- [00:35:06.620]And this sorghum is resistant to herbicide called nicosulfuron.
- [00:35:11.640]The idea behind developing this herbicide resistant sorghum is when it comes to sorghum production,
- [00:35:18.920]we don't really have very good options for post-emergence control of grass weeds.
- [00:35:24.100]It's really challenging.
- [00:35:25.140]So that's why now industry has developed sorghum, which can control grass weeds more effectively.
- [00:35:31.460]Because in sorghum, there is no Roundup-ready sorghum, right?
- [00:35:34.560]So there is no way you can use glyphosate or there is no way you can use some other herbicides like glufosinate,
- [00:35:40.360]which can provide good control of weeds.
- [00:35:43.120]So there are herbicide selection is relatively less when it comes to post-emergence weed control.
- [00:35:48.080]So we did some studies and then John Lindquist
- [00:35:53.760]and Melinda Yerka were also a part of, they were leading this project,
- [00:35:58.000]did some molecular studies on gene flow from sorghum to Johnson grass and setter cane.
- [00:36:04.500]So now we are switching gear.
- [00:36:08.120]We were wanting to use the same concept of pollen movement,
- [00:36:12.760]but now this time it was more about gene flow within crop species.
- [00:36:17.800]So as you know, Nebraska is number one producer of,
- [00:36:23.420]popcorn, right?
- [00:36:25.320]In fact, Nebraska is producing about 45% of popcorn being produced in the country.
- [00:36:30.340]So for example, if there is a field corn and popcorn,
- [00:36:41.040]if they grow side by side,
- [00:36:42.880]do you think popcorn will receive the pollen from field corn?
- [00:36:47.040]No, right?
- [00:36:48.720]Because popcorn is dense, sterile, naturally.
- [00:36:53.280]And that is because of the utilization of this gametophyte factor 1 allyl
- [00:36:59.760]that can prevent the outcrossing or pollen receiving from dense corn to popcorn,
- [00:37:06.740]which is good.
- [00:37:07.960]So popcorn breeder like Dr. Holding is doing those studies.
- [00:37:11.060]He don't need to worry too much about maintaining the isolation distance
- [00:37:15.220]from the corn fields because Nebraska is a husker state, right?
- [00:37:19.000]We have corn everywhere.
- [00:37:20.480]We grow corn on like 10 million acres,
- [00:37:23.020]and we grow popcorn on about 60,000 to 70,000 acres every single year.
- [00:37:28.280]But the problem is this GA1M allyl at the same locus
- [00:37:36.160]can overcome this GA1S dent sterility.
- [00:37:39.160]And most of the Mexican dent corn has that GA1M allyl.
- [00:37:45.780]And a lot of times plant breeders are using different germplasm
- [00:37:49.180]to improve the crop, right, for different traits
- [00:37:52.760]and varieties.
- [00:37:53.680]So North Carolina State University has, by mistake,
- [00:37:58.620]they released 10 inbred lines.
- [00:38:00.480]They were carrying this GA1M allyl.
- [00:38:04.380]So that means if you have that GA1M allyl,
- [00:38:10.860]means your popcorn can receive pollen from dent corn,
- [00:38:14.600]which is problematic, right,
- [00:38:16.100]because then you will not be able to produce good quality popcorn.
- [00:38:22.500]Again, one more student, PhD student, and one more study,
- [00:38:26.560]but now just it was a concept of gene flow,
- [00:38:30.240]but from dent corn, filled corn to popcorn.
- [00:38:35.040]So this was GA1M inbred dent corn,
- [00:38:39.860]and this was based on the studies.
- [00:38:42.680]You can see some yellow color here in this white non-GMO popcorn.
- [00:38:50.160]So this was basically the result
- [00:38:52.240]of the out-crossing and pollen receiving from the dent corn.
- [00:38:57.220]No, actually the dent corn we used was not Roundup Ready.
- [00:39:08.620]It was just carrying this GA1M inbred line
- [00:39:11.860]so that we received from North Carolina State University.
- [00:39:14.560]If there is a Roundup Ready corn growing side-by-side popcorn,
- [00:39:19.960]it will not give
- [00:39:21.980]pollen to the popcorn
- [00:39:23.480]because it is not carrying this GA1M allele.
- [00:39:26.680]It was a specific variety that we received from North Carolina State University
- [00:39:31.080]that was receiving this GA1M allele.
- [00:39:34.580]And that's why it was our popcorn was receiving that gene.
- [00:39:39.680]But fortunately, it is not introduced in the commercially available popcorn hybrids.
- [00:39:48.680]But if it is there, yeah, then it is a problem.
- [00:39:51.720]It is problematic because then this dent corn can also cause problem with popcorn industry.
- [00:39:58.580]And we don't want to see that because popcorn is an important crop in Nebraska.
- [00:40:03.200]So for more information, yeah, we have done this study and it is published in Crop Science.
- [00:40:09.500]One more concept of using this gene flow study.
- [00:40:14.500]Inogen corn came to the market before a few years, developed by industry.
- [00:40:19.500]It's a genetically engineered.
- [00:40:21.460]Corn hybrid, especially for MLS enzyme, alpha MLS enzyme.
- [00:40:28.860]The role is it can convert the starch to sugar more efficiently than traditionally available corn hybrids, which is useful when it comes to ethanol industry, right?
- [00:40:41.880]A lot of corn we produce goes to the ethanol industry for production of ethanol fuel, which can make this process more efficient.
- [00:40:51.440]It takes less time for the fermentation when you have inogen corn.
- [00:40:55.940]So a lot of growers in Nebraska have adopted inogen corn with a contract with Syngenta and they are growing this.
- [00:41:04.420]But the problem is when this pollen-mediated gene flow occurs from inogen corn to non-GMO white corn, food grade corn.
- [00:41:15.260]And again, you see Nebraska is number one producer of this non-GMO food grade white corn.
- [00:41:21.420]We have, I think, about 25,000 acres in Nebraska, which are being grown mostly in contract with a lot of companies.
- [00:41:29.980]They produce food like tortilla and chips and all those food grade white.
- [00:41:37.920]So then again, it was a problem, actually.
- [00:41:41.820]And it was like white corn buyer.
- [00:41:44.640]They have a zero tolerance if they will see any LL coming from inogen corn.
- [00:41:49.540]And the problem is.
- [00:41:51.400]It can break the quality of starch in the white corn.
- [00:41:55.860]So you cannot basically produce tortilla and chips out of those inogen corn gene that received by white corn.
- [00:42:06.580]If Roundup Ready corn will outcross with white corn, there is no problem because it will not interfere with the starch quality.
- [00:42:14.200]But when inogen corn, because it is genetically engineered to have that particular alpha.
- [00:42:21.380]MLS enzyme, which can improve the efficacy for the biofuel production.
- [00:42:26.660]But then unfortunately, it can create the problem and it can break the starch quality in white corn.
- [00:42:34.560]So then you cannot use basically for that purpose.
- [00:42:37.940]And there are a lot of articles are there.
- [00:42:41.420]If you see this Brown Winfield Ag News, they have interviewed a lot of growers and these are the quote I have just taken.
- [00:42:51.360]From those articles. So this was an issue actually in Nebraska, because we have Inogen corn is being adopted by growers because it can give you some more premium price compared with just growing field corn.
- [00:43:05.160]Because you are growing directly in contract with industry and you will get somewhere between like half a dollar to $1 per bushel more premium for producing those.
- [00:43:15.900]And same thing with white corn. So the existence of both of them was an issue.
- [00:43:21.340]So again, one more student, Mandip Singh is here. He completed his PhD leading this project.
- [00:43:27.800]Now, our yellow corn was in the center and then white corn was in the surrounding all the directions.
- [00:43:35.820]And we were wanted to see how far the pollen from Inogen corn can outcross with this white corn.
- [00:43:42.060]And what is the role of wind and wind speed and all those factors that we have done in previous studies.
- [00:43:48.420]So we are using the same concept. But now again,
- [00:43:51.320]this is just to solve some real world problem to see how much actually the minimum isolation distance you have to create
- [00:43:59.260]to avoid this pollen movement from yellow corn to white corn.
- [00:44:05.220]And Mandip, hardworking Mandip here, he has screened like more than 12 million kernel screens, right?
- [00:44:13.280]Because we were harvesting all those seeds and then he needs to make sure he will just identify those yellow kernels
- [00:44:21.300]in this white corn.
- [00:44:23.640]So a lot of hard work and great work.
- [00:44:27.540]In this case, on an average, we were able to see like 2.73% of outcrossing on an average.
- [00:44:35.440]And at 70 meter distance, we have seen about 0.3% of outcrossing, which is still a significant number, right?
- [00:44:41.820]If you are a white corn grower, this is a concern for them.
- [00:44:45.320]And Syngenta has only like 30 feet of isolation distance.
- [00:44:51.280]Which was not really enough to prevent this outcrossing.
- [00:44:54.980]And so based on this study, we have contacted them saying you have to keep at least like 150 feet of isolation distance to reduce this outcrossing.
- [00:45:04.340]And they actually never provided us seeds of Inogen corn.
- [00:45:09.240]They were concerned about their market and business.
- [00:45:12.020]Because I am sure when these results will come out, everyone will see.
- [00:45:16.680]So we use just herbicide resistant Enlist corn just as a concept.
- [00:45:21.260]But if you use the Inogen corn, it will also produce the same level of pollen and it will
- [00:45:27.300]outcross the same way.
- [00:45:28.620]It just we were not able to use the Inogen corn because Syngenta never provided us those
- [00:45:33.880]seeds.
- [00:45:34.160]And those seeds are not available in the marketplace.
- [00:45:36.420]You just have to make a contract with Syngenta and then they will provide us.
- [00:45:41.980]But they were not comfortable to provide us seeds.
- [00:45:45.820]So this was an important study.
- [00:45:48.560]And you can see these are the yellow color.
- [00:45:51.240]You can see in this white corn means, yeah, whatever this happened was because of the movement of the pollen.
- [00:45:56.820]And for this study, we were maintaining an isolation from any other type of corn up to like thousand feet
- [00:46:03.980]because we were not wanted to see any pollen receiving from other corn fields.
- [00:46:09.540]So we were able to do this one study at South Central Ag Lab and another study we did at our NRAC facility near Mead.
- [00:46:21.220]This paper is also published for more information on this topic and thanks Mandeep for leading this project.
- [00:46:29.600]So now we did this gene flow studies for last almost 10-12 years now and we presented in our weed science society meetings.
- [00:46:42.560]So weed science society of America invited me to write
- [00:46:51.200]like a review paper which can provide like overall synthesis of what is going on in this gene flow studies.
- [00:46:57.580]And so I was leading this paper with some of my colleagues and this was during the COVID times when I was traveling relatively less for extension meetings.
- [00:47:07.660]And I was able to finish this project. It took me almost like a one year to come up with this paper.
- [00:47:14.980]In this paper, basically, we were targeting the six weed species and we have provided really
- [00:47:21.180]whatever the information is available about reproductive biology and what are the chances of
- [00:47:26.060]resistant LL transfer due to the pollen movement. So this paper was like a state of the art piece of excellence
- [00:47:34.260]that I have just dissolved myself to finish this chapter. And within a few months when this paper was available online
- [00:47:43.640]for free download on our Weed Science Society of America website, we received so many citations and so the
- [00:47:51.160]society again asked me to write another chapter that can focus on grass weed species.
- [00:47:58.560]So I was leading that chapter one more time, which took me almost like a year.
- [00:48:04.740]We were focusing on these six most terrible grass weeds, which are common in corn and soybean production,
- [00:48:12.880]and also in canola and wheat production in Canada in collaboration with my colleagues over there.
- [00:48:21.140]If you don't want to know anything or if you want to know something about pollen-meditated gene flow studies,
- [00:48:26.900]these two papers are kind of a synthesis of everything that includes the majority of the studies on this topic.
- [00:48:37.580]Summary. This is something important when it comes to gene flow study.
- [00:48:43.040]You really have to have a good PhD student who is willing to take this challenge because gene flow studies are not easy.
- [00:48:51.120]Once you do the study, a lot of statistical analysis, modeling part is there.
- [00:48:55.800]And I was really fortunate to have a very great graduate student working on those projects that provided leadership on those type of studies.
- [00:49:07.860]And we were able to publish all of them like our success rate was like 100 percent.
- [00:49:13.160]Reproductive biology of the weed species is depending on what kind of weed is there, right, if it is diocese species, more chances of gene flow.
- [00:49:21.100]Like waterhemp and palmer amaranth compared with some other species like common lem squatters, which is a self pollinated species.
- [00:49:28.080]So we have seen relatively less chance of gene flow.
- [00:49:35.120]Now, we have a lot of multiple herbicide resistant waterhemp and palmer amaranth, and I believe that is not because of the herbicide selection pressure,
- [00:49:42.540]but it is because of the pollen movement within and between population is floating around in the nature, and that's why we have now
- [00:49:51.080]even in Nebraska, we have palmer amaranth resistant to atrazine and glyphosate and HPPD group of herbicides.
- [00:49:57.700]We have waterhemp resistant to glyphosate, HPPD, and ALS-inhibiting herbicides, so this multiple herbicide resistance
- [00:50:05.720]do not really come from the selection pressure, but I am sure this pollen movement is playing a huge role here,
- [00:50:12.080]but unfortunately, we don't see this, and if you are an early-career faculty, I would say you may need to choose one
- [00:50:21.060]topic that should be kind of out of the box, and that can make you an expert when you see your career after 10 years being in academics.
- [00:50:32.520]Whatever topic you choose, it is good to be an expert of something rather than.
- [00:50:39.240]To be an expert of nothing. Well, I'm really thankful to all my graduate students and my wife and all the support they have provided.
- [00:50:51.040]I greatly appreciate that. Yeah, around the last 12 years, we completed a lot of studies on this topic.
- [00:50:58.720]And there are a number of other topics like management of resistant weeds, which was a major issue.
- [00:51:04.040]And we provided all those research and extensive leadership.
- [00:51:09.160]So, yeah, thank you very much for listening to me so carefully.
- [00:51:13.600]We have time for questions. Yes, sir.
- [00:51:18.260]What's the proportion of male to female seeds?
- [00:51:21.020]For waterhemp? Yeah, yeah.
- [00:51:24.300]Yeah, that's a good question. And the question is, what is the ratio of male to female in
- [00:51:30.000]weed species like waterhemp and palmer emirate?
- [00:51:34.680]Usually it is close to 50 to 60 percent.
- [00:51:37.900]Yeah, even in greenhouse study, for example, we randomly choose seeds right when we choose
- [00:51:43.440]the seeds to do a project in greenhouse.
- [00:51:46.360]We don't know whether the plant is going to be male or female based on the seed.
- [00:51:51.000]Right.
- [00:51:52.000]But the seeds look same.
- [00:51:53.120]Even the plant will look same.
- [00:51:55.020]And when we planted them in the greenhouse, usually we have seen like almost 50 percent
- [00:52:00.840]male and 50 percent female.
- [00:52:03.240]I don't know how that comes.
- [00:52:04.660]But yeah, we have done a number of studies in the greenhouse and even in the field.
- [00:52:08.720]When we are walking in the field, when you see male plant, you will also see female plants.
- [00:52:13.320]So usually they are in a pretty good ratio.
- [00:52:18.120]One more if I might.
- [00:52:19.980]Yeah.
- [00:52:20.980]What is the potential to generate male sterile amaranth or these plants like we do with corn
- [00:52:29.280]and those plants?
- [00:52:30.620]Right.
- [00:52:31.620]Yeah.
- [00:52:32.620]So that's a good question.
- [00:52:33.620]I'm repeating the question.
- [00:52:34.620]So the question is like, can we create like a male sterile plant of Palmer and like pollen?
- [00:52:41.980]And that is true.
- [00:52:42.980]There is a company, it's an Israel based company.
- [00:52:46.940]They are working on developing a sterile pollen for Palmer amaranth.
- [00:52:50.960]So, we had a project with them during the COVID time, we were about to do the studies,
- [00:52:57.760]but then COVID hit and then they were not able to travel to the US, but they are working
- [00:53:03.300]on that direction where they have developed a sterile Palmer amaranth pollen.
- [00:53:09.620]And their idea is when the female plant of Palmer amaranth is ready to flower, you have
- [00:53:15.640]to spray those sterile pollen on that plant.
- [00:53:20.940]So before the plant will receive the other pollen from the natural population, the sterile
- [00:53:26.760]pollen is very aggressive and active.
- [00:53:29.960]It will fertilize the plant and it will basically about the seed production.
- [00:53:35.440]So that is in the process and hopefully we will have something that can be used at commercial
- [00:53:41.700]scale and they were very successful actually.
- [00:53:45.000]Until now they were using just a pollen in a powder form, which is more complicated for
- [00:53:50.920]example, they have a sprayer.
- [00:53:53.100]If something is available in liquid form, they can spray just like they are spraying
- [00:53:57.560]pesticides by using big sprayer.
- [00:53:59.780]But if you have to spray the powder, yeah, there is not a good way.
- [00:54:03.300]So last time I was in Israel for about a couple of weeks, I was visiting this company and
- [00:54:09.740]now they are making progress to develop a liquid formulation of that sterile pollen.
- [00:54:14.660]And how bad, how best the pollen can still remain active until they will mix with the
- [00:54:20.900]water or some other solution and then spray.
- [00:54:23.540]So there is a work being ongoing on that direction and it will be really a good biological solution
- [00:54:30.180]because otherwise, yeah, the way this waterhemp and Palmer is widespread and they are evolving
- [00:54:36.340]resistant to all the herbicides, we are kind of losing battle.
- [00:54:40.840]Yes, sir.
- [00:54:43.260]So waterhemp and Palmer amaranth are technically different species, correct?
- [00:54:48.880]Yes.
- [00:54:49.880]Yeah.
- [00:54:50.880]They are different species.
- [00:54:51.880]In fact, they have different chromosome numbers.
- [00:54:53.900]And they produce a fertile F1 hybrid.
- [00:54:56.380]Right.
- [00:54:57.380]Right.
- [00:54:58.380]So this gets to the whole definition of what constitutes different species.
- [00:55:01.300]Right.
- [00:55:02.300]And they both are still in evolution because being an obligate outcrossing, yeah, I don't
- [00:55:07.240]think they have completed their domestication at all.
- [00:55:10.120]And that's why even chromosome numbers, for example, waterhemp has confirmed chromosome
- [00:55:14.960]number 2N is 32, but when it comes to Palmer amaranth, some studies says it has 32.
- [00:55:20.860]Chromosome numbers and some says like 34 and it used to be like a tetraploid in the past.
- [00:55:27.900]So yeah, so they're both dioecious species.
- [00:55:33.140]So my question is, is the transferability between waterhemp to Palmer amaranth, is that
- [00:55:42.600]the same level of efficiency as within each species or I mean, would it be worth doing
- [00:55:50.840]that experiment with the same species to know, I mean, is it like a lot less efficient?
- [00:55:57.660]Is there a penalty between the two subspecies or not?
- [00:56:02.600]So in our study, what we have seen when we used waterhemp as a male donor, we have seen
- [00:56:09.760]a lot more success of outcrossing with Palmer amaranth and the hybrid being produced and
- [00:56:15.740]then the plant will be fertile.
- [00:56:20.300]So there are more.
- [00:56:20.820]More chances when we have taken waterhemp as a male compared with Palmer amaranth when
- [00:56:25.900]we use Palmer amaranth.
- [00:56:27.200]I think something is with the time when meiosis happens because of little bit of difference
- [00:56:33.020]in chromosome count.
- [00:56:34.640]I think that might be a reason.
- [00:56:36.500]But in future, yeah, that will be a good study if we can do and see this complex between
- [00:56:43.400]amaranthus species hybridization.
- [00:56:45.860]And then the second part, obviously you have a slight decrease in pollinators.
- [00:56:50.800]In transferability in your 50 meters.
- [00:56:53.960]But your experiment really showed that's nowhere near enough.
- [00:56:57.040]Right.
- [00:56:58.040]Right.
- [00:56:59.040]So how, I mean, I was trying to think of an experiment you could design to see the really
- [00:57:02.040]long range transferability of this pollen.
- [00:57:06.020]But the bottom line is what's the given that it only takes one pollen, you know, one successful
- [00:57:11.580]thing on a, on a plant to be, to carry that dominant allele.
- [00:57:17.320]What's the, you know, I mean, you said the application was that.
- [00:57:20.780]Farmers can really know what's the male and female and how and know how to get rid of
- [00:57:26.020]them.
- [00:57:27.020]But what's the, you know, is there, is there hope for stopping this, this spread given
- [00:57:32.780]how far, I mean, probably unless we figure it out, how to utilize this sterile pollen
- [00:57:40.160]and use it at a landscape level, then we can reduce this outcrossing and gene flow and
- [00:57:44.980]seed production.
- [00:57:46.420]That is the only hope, but otherwise, yeah, this pollen movement.
- [00:57:50.760]Hard to control and it is also hard to do like a landscape level study.
- [00:57:55.940]So instead of doing that study, we are just using some prediction model based on our study.
- [00:58:01.600]Like we have seen like up to 5% of outcrossing at 50 meter distance and just using some model
- [00:58:08.000]by working with some computer and mathematical scientists, if we can do and some hypothetical
- [00:58:15.320]equations we can create and see what will be the distance where it will be like.
- [00:58:20.740]But otherwise, now the problem is waterhemp and palmer eminence are so widespread, it's
- [00:58:26.900]been there in each and every county in Nebraska.
- [00:58:30.460]And it can, yeah, it can, wind is favoring the pollination and Nebraska is always windy.
- [00:58:36.540]So yeah, it is hard to find an area where you can have, for example, you can say this
- [00:58:41.200]is 10 acre of the field where there is no any other waterhemp or palmer eminence in
- [00:58:47.060]that area where you can do like a gene flow study at a landscape level.
- [00:58:50.720]So, it will be more challenging to do it practically, but it will be more easy to do it just by
- [00:58:57.220]using some modeling approach.
- [00:59:02.440]Even by doing that landscape level, we will not able to solve this problem easily, right?
- [00:59:07.220]Unless we have that sterile pollen come to the market and it's being available for growers
- [00:59:13.780]where they can just spray everything.
- [00:59:17.560]But now we are also working with some companies like John Deere.
- [00:59:20.700]So now they have developed a combine that has a set up in a way like when the seeds
- [00:59:29.740]of waterhemp and Palmer eminence will go in the combine, the combine has capacity to crush
- [00:59:35.620]them in a powder form.
- [00:59:37.560]So before they go back to the soil, you are like crushing the seeds in a very fine, minute
- [00:59:43.840]powder form.
- [00:59:44.840]So the seeds are gone.
- [00:59:46.680]So you will not have those seeds going back to the soil.
- [00:59:50.680]The seeds are dispersed naturally by the plant.
- [00:59:54.620]Some level of shattering is possible, but they are pretty intact in the female plant.
- [00:59:59.580]So if we will be successful, we have been successful in some of the studies we have
- [01:00:04.560]done in working with John Deere.
- [01:00:07.200]Some of my colleagues are working with their counterpart.
- [01:00:11.280]But this process is very successful in Australia, where Carrington seed destructor is available
- [01:00:17.640]in the marketplace and a lot of grain producers in Australia.
- [01:00:20.660]They are using that system that can just harvest all the wheat seeds at the end of the season
- [01:00:27.260]and it can crush in the powder form.
- [01:00:30.080]So you are not basically adding all the seeds back to the soil.
- [01:00:35.280]So hopefully we will have that in future.
- [01:00:38.040]So we just need to find out this type of solutions, either finding out a sterile pollen or doing
- [01:00:43.780]more work with machinery so all the seeds do not go back to the soil.
- [01:00:50.640]Thanks, everybody.
- [01:00:51.640]Please give one more courtesy to Ahmed.
- [01:00:52.640]Okay.
- [01:00:53.640]Well, thank you, everyone.
- [01:00:54.640]And we have some --
- [01:00:55.640]Okay.
- [01:00:56.640]Well, thank you, everyone.
- [01:00:57.640]And we have some --
- [01:00:58.640]Okay.
- [01:00:59.640]Well, thank you, everyone.
- [01:01:00.640]And we have some --
- [01:01:01.640]Okay.
- [01:01:02.640]Well, thank you, everyone.
- [01:01:03.640]And we have some --
- [01:01:04.640]Thank you.
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