Amyloid Precursor Protein Production and Zika Virus
Brynn Boes
Author
07/28/2021
Added
13
Plays
Description
Amylase Protein Production and Zika Virus
Searchable Transcript
Toggle between list and paragraph view.
- [00:00:01.710]Hello, my name is Brynn bass, and this summer I've been working under Dr. Lewin,
- [00:00:05.970]Shawn. Um,
- [00:00:07.350]and today I will be presenting my presentation entitled amyloid
- [00:00:11.850]precursor, protein production, and Zika virus. So,
- [00:00:16.770]first of all, I'm going to give a bit of an introduction to the Zika virus. Um,
- [00:00:21.330]the Zika virus is a flavor of virus and is thus related to all other flavor
- [00:00:26.320]viruses, such as the dang gay and the west Nile virus. Um,
- [00:00:31.470]the Zika virus is typically transmitted via ATUs mosquito. However,
- [00:00:36.030]it can also be transmitted through sexual contact, blood transfusion,
- [00:00:39.510]and from mother to fetus. Um,
- [00:00:41.640]only about 20% of infected individuals show symptoms. Um, however,
- [00:00:46.320]when the virus is transmitted from mother to fetus, um,
- [00:00:50.940]it can affect the fetus's neural progenitor cells and their neural stem cells,
- [00:00:55.950]which can lead to cause microcephaly microcephaly is a neuro-development
- [00:01:00.840]neurodevelopmental disorder that causes reduction in brain size.
- [00:01:04.590]And this also leads to intellectual disability.
- [00:01:08.160]There is currently no treatment or vaccine for it. Does he get virus
- [00:01:14.120]next?
- [00:01:14.540]I'm going to give an introduction to amyloid precursor protein or otherwise
- [00:01:18.920]known as app. Um,
- [00:01:21.140]app is a transmembrane protein found in brains.
- [00:01:24.830]It is once it is used up, it is metabolized into smaller proteins,
- [00:01:29.060]such as amyloid B by B secretaries.
- [00:01:31.730]One also known as base one and gamma secretase, um,
- [00:01:36.200]clumps of amyloid beat form plaques on the brains of individuals with
- [00:01:40.130]Alzheimer's disease.
- [00:01:41.300]And it is believed though not proven to be a cause of the disease.
- [00:01:46.250]Um,
- [00:01:47.030]there have been structural similarities found between base one and the
- [00:01:51.740]protein in Zika virus.
- [00:01:53.930]And this makes Zika virus a potential candidate to bind to
- [00:01:58.460]app because of its structural similarities. Um, with B one
- [00:02:05.630]next,
- [00:02:06.020]I'm going to talk about app and the Zika virus as they relate to one
- [00:02:10.670]another. Um,
- [00:02:12.380]when Zika virus and app are bound together, uh,
- [00:02:16.460]protein stability of app increases.
- [00:02:19.490]This also causes an increase of app production as a whole.
- [00:02:24.350]Um,
- [00:02:25.460]and app is beneficial to the cell because it acts as a decoy
- [00:02:29.840]receptor for Zika virus. So while on the surface of the cell,
- [00:02:34.940]um, app interacts with the virus,
- [00:02:37.760]which prevents the virus from actually reaching the inside of the cell where it
- [00:02:41.480]can replicate and further infect, um, the victim,
- [00:02:46.280]if sufficient app is not present on the surface of the
- [00:02:50.960]cell and the virus is able to invade the cell app levels will
- [00:02:55.370]decrease. And this has been the main focus of our study, this,
- [00:03:01.410]so our methods of testing this out, um,
- [00:03:06.060]all present next, uh, we started off our, um,
- [00:03:11.970]process with incubating, um, mouse cells with the Zika virus,
- [00:03:16.950]um, and varying amounts.
- [00:03:19.230]And then after the incubation period,
- [00:03:22.230]we use the Western blot technique to analyze the
- [00:03:27.090]prevalence of app in that certain sample. Um,
- [00:03:31.050]for those unfamiliar,
- [00:03:33.750]with the Western blot technique,
- [00:03:36.510]it is a technique in which a gel is utilized, um,
- [00:03:41.310]in a gel electrophoresis method to
- [00:03:45.750]analyze the molecular weight of proteins in the sample,
- [00:03:50.430]thus analyzing the abundance of a certain protein. Um,
- [00:03:56.370]so we were able to analyze the prevalence of app after,
- [00:04:01.500]um, it is, it was exposed to a certain amount of Zika virus. Um,
- [00:04:06.750]this is an example of some results from this procedure. So as you can see,
- [00:04:11.640]especially between the B3 and the before lanes,
- [00:04:15.150]the app level is drastically increased. Um,
- [00:04:20.190]if you look down at the bottom band where it's his tubulin, um,
- [00:04:25.050]since those are relatively the same,
- [00:04:27.840]we can infer that there is much more app in the before sample than there
- [00:04:32.520]is in the beat.
- [00:04:36.510]So our results, um,
- [00:04:39.660]we know that we have known from previous studies that the introduction of Zika
- [00:04:43.890]virus to a cell causes the app to increase because it acts as a
- [00:04:48.720][inaudible] receptor to block the virus from entering the cell altogether.
- [00:04:53.490]Um,
- [00:04:54.690]and we were able to find that one seek a virus has reached the inside of the
- [00:04:59.550]cell app level decreases. Um,
- [00:05:02.730]this is because it is no longer a viable option to stop the spread of the virus
- [00:05:07.260]once it has reached the inside of the cell.
- [00:05:12.360]So here are my acknowledgements
- [00:05:16.770]and here are the references use.
- [00:05:21.060]And thank you for watching my presentation.
The screen size you are trying to search captions on is too small!
You can always jump over to MediaHub and check it out there.
Log in to post comments
Embed
Copy the following code into your page
HTML
<div style="padding-top: 56.25%; overflow: hidden; position:relative; -webkit-box-flex: 1; flex-grow: 1;">
<iframe
style="bottom: 0; left: 0; position: absolute; right: 0; top: 0; border: 0; height: 100%; width: 100%;"
src="https://mediahub.unl.edu/media/17462?format=iframe&autoplay=0"
title="Video Player: Amyloid Precursor Protein Production and Zika Virus "
allowfullscreen
></iframe>
</div>
Comments
0 Comments