Morrison Microscopy Core Virtual Tour Video

Nebraska Center for Biotechnology Author

06/02/2021 Added

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Virtual tour of the Morrison Microscopy Core Research Facility. The Morrison Microscopy core has state of the art imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes.

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[00:00:00.558](upbeat music)
[00:00:07.820]Welcome to the Morrison Microscopy
[00:00:10.310]Research Core Facility.
[00:00:12.410]Located in the first floor of Beadle Center.
[00:00:16.380]This facility provides advanced imaging instrumentation
[00:00:19.590]including confocal laser scanning, transmission,
[00:00:23.320]and scanning electron microscopes.
[00:00:26.460]We also provide expert technical support and services
[00:00:30.460]to researchers from both academic and industrial communities
[00:00:35.220]in the State of Nebraska,
[00:00:38.220]and occasionally from other states in the US.
[00:00:42.730]First of all, we'll talk about the Nikon Ti-2
[00:00:45.560]Inverted Microscope.
[00:00:47.860]This is a latest model of advanced
[00:00:51.220]fluorescence microscope
[00:00:53.100]with a computer control automated stage.
[00:00:57.040]The microscope has a variety of LED filters
[00:01:00.930]covering wavelengths ranging from 350 nanometer
[00:01:05.660]to 700 nanometers.
[00:01:09.220]It can be programmed for collecting images
[00:01:12.150]from multiple wells of a culture plate
[00:01:16.370]or a larger size samples.
[00:01:18.870]This is a confocal laser scanning system
[00:01:21.760]on upright fluorescence microscope.
[00:01:25.190]Which has a motorized stage.
[00:01:28.770]This confocal has six laser lines
[00:01:31.510]and the sensitivity detectors.
[00:01:34.070]Using Nikon elements
[00:01:36.750]advanced imaging programs,
[00:01:39.700]user can study multiple labeled live samples
[00:01:43.400]with the fluorescence protein tags
[00:01:46.250]or immunofluorescence using antibodies.
[00:01:51.140]Since it is a upright confocal,
[00:01:54.380]it is suitable for imaging analysis using uneven surface
[00:01:59.110]such as plant leaf or root samples.
[00:02:03.280]Next one is Nikon A1R.
[00:02:05.820]This is another confocal laser scanning microscope
[00:02:10.610]but it's on the Nikon Ti-2 Inverted Microscope.
[00:02:15.770]It is best for live cell imaging analysis
[00:02:19.000]using cultured cells.
[00:02:22.000]You can culture dishes or well-plate.
[00:02:24.980]We can control the temperature and the carbon dioxide.
[00:02:29.340]It is also a powerful tool
[00:02:31.740]for multi-labeling immunofluorescence
[00:02:34.290]and the co-localization analysis using six laser lines
[00:02:39.160]and the 4+1 channel detector system,
[00:02:43.030]as well as a spectrum imaging analysis
[00:02:45.840]up to 32 channels of wavelength.
[00:02:49.540]It has a motorized stage,
[00:02:51.830]thus it can be used for stitching experiment too.
[00:02:55.430]This is a Hitachi Transmission Electron Microscope
[00:02:58.510]or called TEM.
[00:03:00.090]for ultrastructural analysis,
[00:03:03.048]ultrathin sections of biological samples,
[00:03:06.660]or assessment of nanoparticles,
[00:03:09.550]or other fraction of nano structures and the examination
[00:03:13.380]of a negative standard bacteria or virus particles.
[00:03:19.160]It is mostly used for researchers for biological samples
[00:03:23.940]and for diagnostic analysis,
[00:03:27.090]which needed to be processed
[00:03:30.310]and using specific protocols before analysis.
[00:03:34.160]This core provides such services
[00:03:36.610]and it can be trained users,
[00:03:39.060]can train users to operate the scope.
[00:03:42.880]The last system is Hitachi
[00:03:46.816]S4700 Field-Emission
[00:03:49.740]Scanning Electron Microscope.
[00:03:52.150]This is a high-end scanning electron microscope,
[00:03:55.620]which is powerful tool for topographic analysis
[00:04:00.420]at nano-scale levels for biological symbols
[00:04:04.360]and the conductive nano materials.
[00:04:07.410]This microscope magnify samples
[00:04:12.011]from 20x to 500,000x magnification.
[00:04:16.680]It is a user-friendly system
[00:04:19.040]and that we can provide the training for users
[00:04:21.570]so that they can work on a system independently
[00:04:25.430]with their own sample.
[00:04:27.620]The core is also an important part of the program
[00:04:30.760]supporting Nebraska K12
[00:04:33.360]and the undergraduate student education,
[00:04:35.910]as well as graduate students,
[00:04:38.330]and post-graduate trainees for all Nebraska universities.
[00:04:44.020]Please visit our website for more specific information
[00:04:48.000]about our Imaging Systems and Services.
[00:04:51.238](upbeat music)

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Morrison Microscopy Core Virtual Tour Video

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