Evaluation of ozonated water spray for the microbiological decontamination of food contact surfaces

Grace Gatima Mahoro Author

04/06/2021 Added

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This is a video presentation of a poster that talks about the efficacy of commercialized ozonated water bottle spray at reducing microbial counts on food contact surfaces

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[00:00:00.035]Hello everyone.
[00:00:01.740]My name is Grace Gatima Mahoro.
[00:00:03.993]I'm a senior majoring in Integrated Sciences
[00:00:08.070]and with concentrations in Food Science and Technology.
[00:00:12.060]And today I'll be presenting
[00:00:14.200]about the evaluation of ozonated water spray
[00:00:17.220]for the microbiological decontamination
[00:00:19.540]of food contact surfaces.
[00:00:22.290]And so lately one of the issues that
[00:00:24.600]the food industry is facing
[00:00:26.660]is the presence of pathogenic microorganisms
[00:00:31.720]causing foodborne illnesses and also spoilage organisms
[00:00:36.640]that are reducing the shelf life.
[00:00:39.410]And so there has been the need for another alternative
[00:00:43.290]to clean and sanitize and reduce those pathogens
[00:00:48.880]and micro organisms that are causing harm to food.
[00:00:53.370]And one of those alternatives is the presence
[00:00:55.340]of ozonated water that is being used
[00:00:58.820]for both food contact surfaces and food itself.
[00:01:02.330]And that is, the food on which ozonated water
[00:01:08.660]is being used on is meat, poultry produce and others,
[00:01:13.150]and also on other equipment, on food contact surfaces
[00:01:17.220]such as equipments and homemade utensils.
[00:01:20.613]So the objective of this research was to see
[00:01:30.410]and evaluate the efficacy
[00:01:32.540]of commercials ozonated water systems
[00:01:35.300]in help of decontaminating food contact surfaces
[00:01:40.640]and in households, because there's an increase in production
[00:01:45.430]of ozonated water bottle sprays that are sold commercially.
[00:01:50.720]And if those can easily remove micro organisms,
[00:01:56.187]we would like to know, it is important to know,
[00:02:00.140]to validate it's efectiveness and to ensure
[00:02:05.340]that they can really remove the microbiological activities
[00:02:10.740]as it related in their commercials.
[00:02:16.897]So during this study materials that were used
[00:02:21.890]to food contact surfaces
[00:02:25.800]those were the polypropylene cutting boards
[00:02:29.900]and stainless steel chef knives.
[00:02:32.730]And to ensure that there was no
[00:02:34.300]other micro biological activities occurring
[00:02:39.060]on the food surfaces,
[00:02:42.270]the cutting boards were autoclaved
[00:02:44.580]and the knives were flamed sterilized
[00:02:47.950]to remove any other bacterias that might be present
[00:02:51.990]on their surfaces.
[00:02:55.760]And so bacteria suspensions of E.Coli, listeria innocua,
[00:03:01.050]staphylococcus aureus and pseudomonas species
[00:03:06.370]were spotted and collected
[00:03:07.600]onto a hundred centimeter squared area
[00:03:10.040]of the cutting boards and the blades of the knives.
[00:03:12.950]And there were left to attach for 30 minutes
[00:03:15.747]at room temperature.
[00:03:18.562]And then what three water works sanitizing spray bottle
[00:03:23.130]that contains at least
[00:03:24.380]almost 1.5 to 2 PPM ozone concentration.
[00:03:29.450]It was sprayed on the diseminated area
[00:03:33.080]following the manufacturer's instructions.
[00:03:36.360]And it was left to stay there for 30 seconds
[00:03:40.090]and 60 seconds respectively.
[00:03:42.420]The surface was some producing polyurethane sponge samplers
[00:03:48.230]with D/E neutralizing broth.
[00:03:51.580]To see if there was any growth
[00:03:53.240]or any change in bacterial counts
[00:03:55.840]we use macconkey agar for E.Coli,
[00:03:58.430]luria broth agar for pseudomonas species,
[00:04:02.010]brain heart infusion agar for listeria innocua
[00:04:05.030]and tryptic soy agar for staphylococcus.
[00:04:08.370]So they were some untreated areas
[00:04:10.380]and those were used as the control.
[00:04:13.120]And the experiment was performed in Triplicates
[00:04:17.800]And now going to the results as you can see,
[00:04:20.100]we have both a table and a graph that shows
[00:04:24.480]the reduction in microbial counts
[00:04:26.977]and also a table that shows the bacterial counts
[00:04:32.500]in comparison to the time, the control and ozonated
[00:04:38.190]and comparison between board and knife.
[00:04:41.690]And as you can see on the graph there was a difference
[00:04:47.310]in reduction where 30 seconds there was barely any reduction
[00:04:53.960]when it came to the difference
[00:04:59.700]between ozonated treated surfaces and control surfaces.
[00:05:06.933]And when the time that it was applied increased
[00:05:17.930]to 60 seconds, and there was an increase in reduction.
[00:05:21.980]And when we went to board, to the knives
[00:05:25.780]there was also a higher increase.
[00:05:27.570]And that shows that an increase in time suspension
[00:05:33.300]or an increase in time allows the ozone to work
[00:05:38.540]and remove the micro organisms.
[00:05:43.080]So, which means it works better
[00:05:46.060]on the 60 seconds than on the 30 seconds.
[00:05:49.560]So our conclusions that were drawn from these results
[00:05:53.450]were that the exposure time influenced the decrease
[00:05:57.280]in microbial counts and effectiveness of the ozonated water.
[00:06:01.500]Surface material influenced effectiveness
[00:06:04.470]of ozonated water as a greater reduction were achieved
[00:06:08.400]on stainless steel compared to polypropylene, and listeria innocua
[00:06:13.610]showed greater reduction on cutting boards.
[00:06:18.540]And while E.Coli showed greater increase
[00:06:22.860]in reduction on stainless steel.
[00:06:26.050]knives, and that will be all for the presentation.
[00:06:31.620]I would like to thank my supervisor Carmen Cano,
[00:06:35.770]my direct supervisor Carmen Cano
[00:06:37.570]and my mentor Dr. Bryon Chaves
[00:06:40.310]for helping me throughout this research.
[00:06:42.980]And I hope you enjoyed learning
[00:06:45.840]about ozonated water spray and its effect
[00:06:50.890]on decontaminating food contact surfaces.
[00:06:55.890]That will be all for today.
[00:06:57.260]Thank you for watching.

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Evaluation of ozonated water spray for the microbiological decontamination of food contact surfaces

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