Role of Aromatic Residues in WhiB5 for Binding to the Primary Sigma Factor in Mycobacterium tuberculosis

Noah Mettler Author
04/02/2021 Added
13 Plays


Mycobacterium tuberculosis uses Wbl transcriptional regulators to control virulent activity. This research specifically focuses on the role of WhiB5 residues, Trp12 and Phe13, in binding WhiB5 to the primary sigma factor (SigA). The importance of these residues in binding interactions was determined by the use of an in vitro pull-down assay. Furthermore, results were analyzed by spectroscopic and electrophoretic techniques.

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