Biosafety Cabinets

Environmental Health & Safety Author

05/31/2019 Added

227 Plays

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Review of proper operation, use and care of biological safety cabinets

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[00:00:00.834](upbeat music)
[00:00:13.110]The biological safety cabinet is an engineering control
[00:00:16.340]designed to protect both you and the materials handled
[00:00:19.760]from contamination.
[00:00:21.420]This is accomplished by directional air flow
[00:00:24.280]through high efficiency particulate air, or HEPA filters
[00:00:27.800]located within the cabinet.
[00:00:29.800]When properly maintained, and in conjunction with
[00:00:32.240]proper microbiological techniques,
[00:00:34.360]the biosafety cabinet can be a useful tool
[00:00:36.700]in a biological laboratory.
[00:00:38.660]This video will discuss proper operation, use,
[00:00:41.740]and care of the biological safety cabinet.
[00:00:44.470]For more operational information,
[00:00:46.470]please consult the owner's manual of the specific cabinet.
[00:00:49.650]For additional guidance, you may also consult
[00:00:51.980]the EHS SOP biosafety cabinets, located on the EHS website.
[00:00:57.820]Biosafety cabinets must be certified annually
[00:01:00.750]to ensure proper airflow and HEPA filtering.
[00:01:04.270]Check the certification sticker for currency and results.
[00:01:08.030]Do not use a cabinet which has not been certified
[00:01:10.460]within the past year.
[00:01:12.130]Notify the Biosafety Officer if
[00:01:13.960]a cabinet has failed certification.
[00:01:16.940]In addition to annual certifications,
[00:01:19.210]biosafety cabinets must be recertified if moved or serviced.
[00:01:26.110]Before any work in the biosafety cabinet,
[00:01:28.700]it is important to don appropriate PPE,
[00:01:31.210]which often includes lab coat, eye protection, and gloves.
[00:01:35.400]Use of the biosafety cabinet is not
[00:01:37.480]a substitute for poor work practices.
[00:01:40.680]After the air has been moving within the cabinet
[00:01:43.250]for three to five minutes,
[00:01:44.880]decontaminate the workspace with 70% alcohol
[00:01:48.980]and place your work materials inside the cabinet.
[00:01:53.060]As you do so, establish a clean side and a dirty side,
[00:01:57.430]which will help reduce the risk of cross-contamination.
[00:02:01.000]Always work from clean to dirty.
[00:02:03.680]The dirty side should contain a container
[00:02:06.060]for biohazardous waste and/or disinfectant solution.
[00:02:10.050]To allow for proper airflow, you should only place
[00:02:12.820]materials needed for the work session in the cabinet.
[00:02:15.730]Never block the front or back grilles of the cabinet.
[00:02:19.850]All work within the cabinet should be smooth and deliberate,
[00:02:23.510]free from outside distractions.
[00:02:26.210]Avoid rapid arm movements in and out of the cabinet
[00:02:29.580]so as to not disrupt proper airflow.
[00:02:32.540]Also, foot traffic behind or near the cabinet
[00:02:35.680]should be minimized.
[00:02:37.360]Maintain an ergonomic posture and work efficiently.
[00:02:40.770]With few exceptions, a work session
[00:02:42.840]only lasts a few minutes.
[00:02:45.370]It is important to maintain containment
[00:02:47.390]when removing items from the cabinet.
[00:02:49.840]When work is completed, close and remove
[00:02:52.700]any experimental culture flasks or tubes
[00:02:55.410]and place in the appropriate incubator or location
[00:02:58.100]as experimentally determined.
[00:03:00.150]Close and remove any unused clean items from the cabinet.
[00:03:04.370]If necessary, you may spray the lids with disinfectant
[00:03:07.600]prior to removing.
[00:03:09.370]Then, close any containers or bags
[00:03:12.500]containing biohazardous materials.
[00:03:15.140]Also, if necessary, you may spray
[00:03:17.760]the outside of the container or bag with disinfectant
[00:03:21.040]to reduce the risk of cross-contamination.
[00:03:24.520]Carefully remove contaminated items
[00:03:26.850]and place into a larger biohazard waste container
[00:03:30.010]for autoclaving and disposal.
[00:03:32.450]Please refer to the EHS SOP
[00:03:34.930]Autoclave Use and Performance Testing
[00:03:37.230]for additional guidance on disposing biohazardous materials.
[00:03:41.840]In the event of an emergency requiring immediate evacuation,
[00:03:45.630]close the sash, turn off the fan, remove PPE,
[00:03:49.610]wash hands and evacuate the building.
[00:03:52.080]Do not attempt to remove items from the cabinet.
[00:03:55.660]In the event of a mechanical failure of the cabinet,
[00:03:58.260]such as blower or power failure, close the sash immediately.
[00:04:02.500]Do not open the sash until
[00:04:04.070]functionality has been fully restored.
[00:04:07.030]In the event of a large spill within the cabinet,
[00:04:09.520]decontaminate and clean using
[00:04:11.410]the laboratory biohazardous materials spill kit.
[00:04:14.570]Thoroughly decontaminate the spill prior to cleaning it.
[00:04:18.280]Employ assistance of a coworker if necessary.
[00:04:22.150]Be sure to report any illnesses or injuries
[00:04:24.610]to your supervisor and EHS immediately.
[00:04:28.550]Pay attention to any alerts or alarms
[00:04:30.680]which may occur during work sessions.
[00:04:34.150]Sash is too high.
[00:04:36.600]If this alarm sounds (machine beeping)
[00:04:39.970]the sash is open too far for safe operation.
[00:04:43.370]The alarm should silence when
[00:04:44.870]the proper sash height has been attained.
[00:04:47.810]Airflow alert.
[00:04:49.460]This alert occurs when airflow patterns in the cabinet
[00:04:52.500]have changed, resulting in
[00:04:54.070]a sudden change in the motor speed.
[00:04:56.490]This is most likely due to a blockage of the grille
[00:04:59.260]or the exhaust filter outlet.
[00:05:01.540]It may also be caused by removal of the work surface
[00:05:04.780]while the cabinet is in operation.
[00:05:07.460]Airflow alarm.
[00:05:09.010]If equipped with the optional airflow sensor
[00:05:11.380]and the inflow or down flow velocities
[00:05:13.650]are excessively high or low, this alarm will be displayed.
[00:05:17.710]System error.
[00:05:19.000]The motor and display circuit board
[00:05:20.810]are not communicating properly.
[00:05:22.820]Do not use the cabinet until
[00:05:24.360]this problem has been corrected.
[00:05:26.560]Please consult the equipment operator's manual
[00:05:29.140]for additional instructions if these alarms occur
[00:05:31.840]or are persistent.
[00:05:34.080]The demonstration shown in this video
[00:05:35.890]utilized a Labconco cabinet.
[00:05:38.030]The alarms and controls for your cabinet may be different.
[00:05:40.860]Please be sure to consult the user's manual
[00:05:43.040]for information on your specific cabinet alarms
[00:05:45.420]and troubleshooting procedures.
[00:05:47.290]This should be done prior to using the cabinet.
[00:05:51.150]In general, biosafety cabinets are not designed
[00:05:54.160]for use with large quantities of volatile chemicals
[00:05:57.150]or intense heat sources.
[00:05:59.480]In addition, any radioisotope use within the cabinet
[00:06:02.820]requires prior approval from EHS radiation safety.
[00:06:07.060]Please consult the listed EHS SOPs
[00:06:09.610]for additional guidance and information.
[00:06:11.970]This concludes the biosafety cabinet training.
[00:06:15.060]Please indicate completion of this training
[00:06:17.120]in your laboratory biosafety manual.
[00:06:19.819](upbeat music)

Biosafety Cabinets

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