Fragrant Signal 2

Scott Dworak Author

02/12/2019 Added

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This video details the design of two jar1 experiments that would yield information on the relationship between cabbage looper feeding and jasmonic acid production.

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[00:00:00.000]In preliminary studies, Scott determined that the cabbage loopers were mobile and he wanted
[00:00:06.020]to force them to feed on just one type of Arabidopsis plant.
[00:00:10.240]So he established islands of plants of each type by placing the plants in their pots in
[00:00:14.600]a tray of water.
[00:00:16.320]Cabbage loopers taking a swim were removed from the study.
[00:00:19.820]With this set up, he conducted two experiments.
[00:00:22.820]One experiment tested the plant's immediate response to the insect feeding.
[00:00:26.940]In this case, the insect fed for 30 minutes.
[00:00:29.660]Then the plants were sampled to determine how they controlled their stress response
[00:00:33.260]hormone and the signal pathway.
[00:00:36.040]The second experiment was focused on the insect response to the two types of Arabidopsis plants.
[00:00:42.180]If Jar 1 signals help, the Arabidopsis plant mount
[00:00:44.900]of defense. The cabbage loopers might feed less on the normal compared with the mutated plants.
[00:00:50.330]This experiment required a three-day feeding time. The key data collected in experiment number two
[00:00:56.730]was the weight change in the cabbage looper. Scott weighed the loopers before he placed them on the
[00:01:01.930]plant and then after their three-hour feeding period. If their hypothesis was supported by
[00:01:06.970]their experimental results, the weight changes would be different between the cabbage loopers
[00:01:11.370]on normal Arabidopsis compared with the loopers on the Jar 1 mutant types.

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Fragrant Signal 2

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