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-: Welcome to the Flow Cytometry Laboratory.
We are located in North Wing
of the Morrison Life Science Research Center
on the first floor.
For more details on how to find that facility,
please email me.
The laboratory is large and easy to social distance
and we have open bench space available
and a centrifuge for use if users wish
to prepare their samples in the lab.
The center is open for business Monday through Friday
from 8:00 to 5:00,
evening and weekend access may be granted to users
with appropriate training.
At the moment, the center has two primary instruments
to meet researchers needs.
The newest addition to the facility
is the Beckman Coulter CytoFLEX LX analysis system.
The CytoFLEX is equipped with four laser lines
and 16 fluorescence detectors.
It is driven by the intuitive site expert software
and I do offer training on this system
if users are interested in running their own experiments
or I will be happy to run experiments for customers.
In addition to the fluorescent detectors,
it is also equipped with extremely sensitive
forward and side scatter detectors
that are capable of visualizing particles
as small as 300 nanometers.
So it is capable of analyzing extracellular vesicles
and even other nanoparticles.
Most of the work that is done on the system
is looking at multi-parameter fluorescence panels
with an option to load samples into 96-well plate
for automatic acquisition.
It has proven to be the lab's workhorse.
The other main system in the lab
is our BD FACSAria II cell sorter.
This system also has four laser lines working
with 17 fluorescence detectors
and can be used for analysis if necessary.
But the real benefit of this system
is that it is capable of collecting
and purifying specific subsets from the sample.
For example, if you are working with a transfected cell line
and you only want to work with the GFP-positive cells,
we can run the sample through this order
and collect and isolate only the cells
that have expression of GFP
while removing all the negatives.
You could also sort your GFP containing cells directly
into a multi-well plate
as few as one cells per well if needed
and put them back into culture to grow further.
The sorter is housed in the biosafety cabinet
for user and staff protection
and because of that,
we were able to work with BSL-2 Plus level samples.
We also welcome users who are external
to the university to come and use our facilities.
Our prices are listed on our website.
And the lab does offer free consultations
if you would like to discuss
whether the Flow Lab would be useful for your research.
Please feel free to reach out to me
if you have any questions
about experiments that you would like to discuss.
(bright upbeat music)